Damaged DNA-binding proteins: recognition of N-acetoxy-acetylaminofluorene-induced DNA adducts.
نویسندگان
چکیده
منابع مشابه
Excision of A/-Acetoxy-2-acetylaminofluorene-induced DNA Adducts from Chromatin Fractions of Human Fibroblasts1
The excision and persistence of covalent DNA adducts formed by A/-acetoxy-2-acetylaminofluorene (AAAF) were stud ied in human skin fibroblasts. The changes in adduct concen tration as a function of posttreatment incubation were meas ured in purified nucleosomal core DNA and in total nuclear DNA, and from these data the changes in adduct concentration in nucleosomal linker DNA were calculated. I...
متن کاملIn Vitro Binding of N-Acetoxy-N-2-acetylaminofluorene in Chromatin to DNA
The binding of N-acetoxy-N-2-acetylaminofluorene to DNA in native and partially dehistonized chicken erythrocyte chromatin was studied. The amounts of carcinogen bound to DNA were measured, after removal of proteins with phenol, by using the absorption ratio A3os/A260 or by counting the radioactivity of 14C-labeled carcinogen. Measurements of uncovered zones of DNA in chromatin were made by com...
متن کاملAntibodies to DNA modified by the carcinogen N-acetoxy-N-2-acetylaminofluorene.
Several studies have shown that the carcinogen N-acetoxy-N-2-acetylaminofluorene (AAAF) reacts in vivo and in vitro with native DNA and that the DNA contains a major (80%) adduct N(deoxyguanosin8-yl)-acetylaminofluorene (dGuo8-AAF) and a minor (20%) adduct 3{deoxyguanosin-N2-yl)-acetylaminofluorene (dGuo-N2-AAF) (reviewed [1,2]). It has been shown that the major alteration induced by the fluore...
متن کاملDistribution of initial and persistent 2-acetylaminofluorene-induced DNA adducts within DNA loops.
The intranuclear distribution of initial and persistent DNA adducts induced in vivo after four weekly injections of the hepatocarcinogen 2-acetylaminofluorene was examined in rat liver by using a protocol that fractionates chromatin from various regions of each of the multiple nuclear DNA loops. Ten hours after the initial dose, two acetylated [(N-acetyl-N-(deoxyguanosin-8-yl)-2-aminofluorene a...
متن کاملReactivity of B and Z-DNA towards N-acetoxy-2-acetylaminofluorene.
Poly d(G-C) d(G-C) in B-form, on one hand, and poly d(G-br5C). poly d(G-br5C) and poly d(G-m5C) . poly d(G-m5C) in Z-form, on another hand, were treated with N-AcO-[3H]AAF and the kinetics of these reactions were followed by radioactivity. Covalent binding of carcinogen to the polymers was evaluated after separation of the reacted polymers from non-reacted carcinogen by thin-layer chromatograph...
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ژورنال
عنوان ژورنال: Acta Biochimica Polonica
سال: 1999
ISSN: 1734-154X,0001-527X
DOI: 10.18388/abp.1999_4195